Sufferers, who’re handled with indwelling catheters via the urethra, usually have bacterial colonisation of their urine after a time period. Nonetheless, these micro organism may cause infections which might be generally troublesome to deal with and may even be life-threatening.
“Increased antibiotic resistance further reduces the chances of successful treatment, therefore alternatives to traditional antibiotic treatment are an important aspect,” says John Kerr White, researcher on the Division of Microbiology, Tumor and Cell Biology, Solna, Karolinska Institutet, and shared first writer.
Dangers of a Urinary Catheter
A attainable different therapy focuses on the usage of antimicrobial peptides, naturally discovered within the physique. They’ve amongst different issues, antibacterial qualities, however the drawback is their comparatively brief lifespan. Lately, antimicrobial peptides have been synthetically developed to enhance their stability and efficacy towards micro organism, while being innocent to the physique’s personal cells.
Annelie Brauner’s analysis group has studied a particular artificial antimicrobial peptide, CD4-PP, which was developed by professor Ulf Göransson’s analysis group, Uppsala College. Within the research, the researchers examined what impact CD4-PP had on the commonest bacterial strains that trigger urinary tract infections, corresponding to E. coli, Okay. pneumoniae and P. aeruginosa.
“The study shows that CD4-PP has good bactericidal effect against these urinary tract bacteria as well as being effective against antibiotic-resistant bacteria, which can be very difficult to treat,” says Annelie Brauner, professor of Medical microbiology, on the Division of Microbiology, Tumor and Cell Biology, Solna, Karolinska Institutet, and the research’s senior writer.
CD4-PP was additionally proven to be lively towards biofilm, a sort of mucus blanket that micro organism kind, which will increase their resistance to the physique’s immune system in addition to to completely different antibiotics. CD4-PP was discovered to forestall the formation of recent biofilm and likewise dissolved current biofilm. The helpful impact was additional enhanced by the truth that CD4-PP additionally activated the immune system to guard cells towards an infection.
Stopping micro organism from attaching to the catheter itself is a vital a part of lowering the chance of infections.
“When we applied CD4-PP together with a saline fluid on urinary catheters, we found that the adhesion of E. coli to the catheters decreased. Since bacterial adhesion is the first step of the infection process, this effect is important in preventing urinary tract infections. We now plan to further develop and refine how CD4-PP can be applied when using catheters. We will also be investigating how CD4-PP reacts to other types of bacterial strains, such as those that cause infections in wounds”, says Annelie Brauner.